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Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
06/01/2016 |
Data da última atualização: |
20/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GUIMARÃES, A. L. S.; PEREIRA, S. A.; KUSSANO, N. R.; DODE, M. A. N. |
Afiliação: |
A. L. S. GUIMARÃES, UnB; S. A. PEREIRA, UnB; N. R. KUSSANO, UnB; MARGOT ALVES NUNES DODE, CENARGEN. |
Título: |
The effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
Zygote, p. 1-11, 2015. |
DOI: |
10.1017/S0967199415000064 |
Idioma: |
Inglês |
Conteúdo: |
This study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 M) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 M) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 M cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 M) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the |
Palavras-Chave: |
Cilostamide; Cultura pré-maturação; Meiotic arrest; Oócitos bovinos. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182435/1/effect-of-prematuration-culture-using-phosphodiesterase-type-3-inhibitor-and-insulin-transferrin-and-selenium-on-nuclear-and-cytoplasmic-maturation-of-bovine-oocytes.pdf
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Marc: |
LEADER 01878naa a2200217 a 4500 001 2033071 005 2023-03-20 008 2015 bl uuuu u00u1 u #d 024 7 $a10.1017/S0967199415000064$2DOI 100 1 $aGUIMARÃES, A. L. S. 245 $aThe effect of pre-maturation culture using phosphodiesterase type 3 inhibitor and insulin, transferrin and selenium on nuclear and cytoplasmic maturation of bovine oocytes.$h[electronic resource] 260 $c2015 520 $aThis study aims to evaluate if a pre-maturation culture (PMC) using cilostamide as a meiotic inhibitor in combination with insulin, transferrin and selenium (ITS) for 8 or 24 h increases in vitro embryo production. To evaluate the effects of PMC on embryo development, cleavage rate, blastocyst rate, embryo size and total cell number were determined. When cilostamide (20 M) was used in PMC for 8 or 24 h, 98% of oocytes were maintained in germinal vesicles. Although the majority of oocytes resumed meiosis after meiotic arrest, the cleavage and blastocyst rates were lower than the control (P < 0.05). When the cilostamide concentration was lowered (10 M) and oocytes were arrested for 8 h, embryo development was improved (P < 0.05) and was similar (P > 0.05) to the control. The deleterious effect of 20 M cilostamide treatment for 24 h on a PMC was confirmed by lower cumulus cell viability, determined by trypan blue staining, in that group compared with the other groups. A lower concentration (10 M) and shorter exposure time (8 h) minimized that effect but did not improve embryo production. More studies should be performed to determine the 653 $aCilostamide 653 $aCultura pré-maturação 653 $aMeiotic arrest 653 $aOócitos bovinos 700 1 $aPEREIRA, S. A. 700 1 $aKUSSANO, N. R. 700 1 $aDODE, M. A. N. 773 $tZygote, p. 1-11, 2015.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registros recuperados : 27 | |
13. | | GUIMARÃES, A. L. S.; CARVALHO, J. O.; LEME, L. O.; SPRÍCIGO, J. F. W.; PIVATO, I.; DODE, M. A. N. Efeito do cultivo in vitro no tamanho e sexo de embriões bovinos no D14 de desenvolvimento. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 29., 2015, Gramado. Anais... [S.l.]: SBTE, 2015. p. 337.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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14. | | CUNHA, A. T. M.; CARVALHO, J. O.; GUIMARÃES, A. L. S.; LEMEID, L. O.; CAIXETA, F. M.; VIANA, J. H. M.; DODE, M. A. N. Bovine epididymal spermatozoa treatment for in vitro fertilization: Heparin accelerates fertilization and enables a reduction in coincubation time. PLoS ONE, v. 14, n. 1, article e0209692, 2019.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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15. | | ZACARIAS, T. A.; GUIMARÃES, A. L. S.; SCALIANTE, J. R.; RODRIGUES, S. A. D.; DODE, M. A. N.; FIGUEIREDO, R. A. Hormonal stimulation in Nelore calves for in vitro embryo production. In: ANNUAL MEETING OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY (SBTE), 29., 2015, Gramado.; MEETING OF THE EUROPEAN EMBRYO TRANSFER ASSOCIATION (AETE), 31, 2015, Ghent. Proceedings... Animal Reproduction., v.12, n.3, p. 546, Jul./Sept. 2015. A172. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | SPRÍCIGO, J. F. W.; SENA NETTO, S. B.; SIMOES, L. M. S.; LEME, L. O.; GUIMARÃES, A. L. S.; PIVATO, I.; DODE, M. A. N. Transferência intrafolicular de ovócitos imaturos: uma alternativa para a multiplicação de fêmeas bovinas. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 29., 2015, Gramado. Anais... [S.l.]: SBTE, 2015. p. 307.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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18. | | SPRÍCIGO, J. F. W.; GUIMARÃES, A. L. S.; CUNHA, A. T. M.; LEME, L. de O.; CARNEIRO, M. C.; FRANCO, M. M.; DODE, M. A. N. Using Cumulus Cell biopsy as a non-invasive tool to access the quality of bovine oocytes: how informative are they? Animals, v. 12, n. 22, 2022. 3113Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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19. | | SENA-NETTO, S. B.; SPRÍCIGO, J. F. W.; LEME, L. O.; GUIMARÃES, A. L. S.; CAIXETA, F. M. C.; DODE, M. A. N.; PIVATO, I. The replacement of fetal bovine serum with bovine serum albumin during oocyte maturation and embryo culture does not Improve blastocyst quality after slow freezing cryopreservation. Biopreservation and biobanking, v. 18, n. 3, p. 171-179, 2020.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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20. | | KAWAMOTO, T. S.; ZACARIAS, T. A.; GUIMARÃES, A. L. S.; SCALIANTE, J. R.; RODRIGUES, S. de A. D.; FIGUEIREDO, R. A. Serum FSH, AMH related to number and morphology of oocytes in superovulated 4 to 7 months old Nelore breed females (Bos Taurus Indicus). Animal Reproduction, v.14, n.3, p.757, jul./set. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 27 | |
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